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Introduction to Spatial Transcriptomics

Authors: Jason Shapiro, Ph.D. and Diana Vera Cruz, Ph.D.

Topic Materials Type
Overview Slides Slides
Visium Example 1 Seurat vignette Markdown and Rmd
Visium Example 2 Tumor microenvironment Markdown and Rmd
GeoMx QC 1_geomx_setup_qc Markdown and Rmd
GeoMx DE Geomxtools 2_geomx_norm_DE Markdown and Rmd
GeoMx DE standR,limma 2B_geomx_standR_norm_DE Markdown and Rmd
GeoMx DE limma 2C_geomx_limma_norm_DE Markdown and Rmd
GeoMx - Spatial Deconvolution geomx_SpatialDecon Markdown and Rmd
GeoMx - Seurat conversion geomx_Seurat Markdown and Rmd

These materials are provided as part of the workshop Introduction to Spatial Transcriptomics held on April 3rd, 2025, at the University of Chicago. The workshop covers the basics of analyzing data from 10X Genomics' Visium platform and NanoString's GeoMx platform. For additional details on the example code and datasets used for each section, please start from the separate folders for Visium or GeoMx.

This workshop was developed by members of the University of Chicago Center for Reseach Informatics. The associated markdown files are open access materials distributed under the terms of the Creative Commons Attribution license (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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