yeastDrop-Seq Computational Pipeline

This repository contains scripts for data pre-processing and downstream analysis in the yeastDrop-Seq paper.

Dataset is available at GEO viewer (Accession Number: GSE165686)

Required softwares

Data preprocessing

UMI-tools samtools Fastqc STAR Trimmomatic subread

Downstream analysis

SC3 SingleCellExperiment Seurat ggplot2 scales ggpubr cowplot RColorBrewer networkD3 dplyr patchwork reshape2 clusterProfiler org.Sc.sgd.db